研究队伍

Post-doctor

Email：muhammadwasimusmani(AT)nbu.edu.cn

 Research areas/interests

Molecular Pathogenesis & Neuroprotection of Nervous System Disorders

Experimental Animal Models and Behavior Analysis

Molecular epidemiology and pathobiology of infectious diseases from one health perspective

Personal Brief Introduction

The main research area is evaluating mechanism-based pharmacological activities of Chinese herbal medicines to enhance learning & memory, overcome depression, and neuroprotection in animal models. Insights into the biological mechanisms, i.e., modulation of neurochemical pathways and reducing inflammatory markers to attenuate chronic sleep deprivation-induced dysfunction. Previously, I worked on the Molecular epidemiology and pathobiology of pox virus disease in ruminants, application of an animal disease model to study the immunopathological changes against pathogen-derived host-immune response, serological assays, and immunomodulation by coconut oil extract and essential oils in poultry birds. Furthermore, I have hands-on expertise in basic immunological and molecular assays such as isolation, propagation, and molecular characterization of pathogens from animals and their products, understanding gene expression/signaling pathways, fluorescence microscopy, ELISA, immunohistochemistry, and histopathology.

Education Experience

2009.07-2014.07

University of Agriculture Faisalabad, Doctor of Veterinary Medicine (DVM)

2014.09-2016.10

University of Agriculture Faisalabad, M.Phil. Pathology

2017.01-2022.08

University of Agriculture Faisalabad, Ph.D Pathology

2019.04-2022.01

Department of Pathology, UAF, Research Assistan

2022.12-2024.12

Ziauddin University Karachi, Assistant Professor

2024.09-Present

Institute of Drug Discovery Technology, Ningbo University China, Postdoctoral Fellow

Funding and Projects

Worked as a Research Assistant in a National Research Program for Universities (NRPU)-Higher Education Commission (HEC) Pakistan, Molecular epidemiology of buffalo pox in Pakistan, comparison of conventional and molecular diagnosis, polypeptide mapping, restriction enzyme analysis and sequence homology, Project No. 20-7590/R&D/NRPU/2018, 2.96million PKR.

 Research Paper Published

1. Usmani MW, Rizvi F, Saleemi MK, Ahmad MZ, Numan M, Shakir MZ, Mahmood N, and Tahir J, 2024. A study of the immunomodulatory effects of coconut oil extract in broilers experimentally infected with velogenic Newcastle disease virus. Anim Biosci, 37(10):1809-1819. https://doi.org/10.5713/ab.23.0489

2. Masoud F, Ashraf MA, Usmani MW, Rafique A, and Aslam R, 2024. Newcastle disease virus as a viral vector platform for poultry vaccines: a review. Pak Vet J, 44(3): 539-546. http://dx.doi.org/10.29261/pakvetj/2024.217

3. Haq SU, Khalid A, Meghwar KH, Khan A, Siddiqui SA, Ullah R, Sadeeq M, Usmani MW, and Ashfaq K, 2024. Exploring the hepatoprotective effects of silymarin in experimentally induced-diclofenac sodium toxicity in rabbits. World Journal of Biology and Biotechnology, 9(1):21-24. https://doi.org/10.33865/wjb.009.01.1207

4. Aziz D, Qureshi ZI, Waqas MS, Ijaz M, Hussain I, and Usmani MW, 2023. Comparative efficacy of GnRH, HCG and seminal plasma for induction of ovulation during the breeding season in the dromedary camel (Camelus dromedarius). Pak Vet J, 43(4): 809-813. http://dx.doi.org/10.29261/pakvetj/2023.095

5. Ahmad S, Haq SU, Hussain S, Ashfaq K, Siddiqui SA, Khan A, Yameen A, Usmani MW, Ullah R, and Aslam MA, 2024. Comparison of photochemical tissue bonding using rose bengal dye and conventional suturing for closure of incisional cutaneous wounds in canine model. Sarhad J Agric, Accepted. http://dx.doi.org/10.17582/journal.sja/xxxx.

6. Ashfaq K, Haq SU, Tehreem A, Rahim A, Saddiqui SA, Ullah A, Taj R, Usmani MW, and Khan A, 2023. Hepatoprotective outcome of silymarin against CCl₄ induced hepatotoxicity in rabbit model. World Journal of Biology and Biotechnology, 8(2):33-37. https://doi.org/10.33865/wjb.008.02

7. Usmani MW, Rizvi F, Khatoon A, and Mahmood MS, 2022. Seroprevalence, associated risk factors and clinico-pathological studies of buffalopox disease in various regions of Punjab province, Pakistan. Pol J Vet Sci, 25(1):137–147. https://doi.org/10.24425/pjvs.2022.140850

8. Amjad N, Rizvi F, Shakir MZ, Usmani MW, Amjad MR, and Khalid MA, 2022. In vivo concurrent efficacy of purified Immunoglobulins and Licorice (Glycyrrhiza glabra) root extract against Newcastle Disease Virus. Pak J Agric Sci, 59(6):985-992.https://doi.org/10.21162/PAKJAS/22.874

9. Wattoo F, Roheen T, Saleem K, Rizvi F, Aslam S, and Usmani MW, 2022. Latest trends in Diagnosis of Tuberculosis: A review. The Professional Medical Journal, 29(8):1106-1111. https://doi.org/10.29309/TPMJ/2022.29.08.6690

10. Shakir MZ, Rizvi F, Saleemi MK, Usmani MW, Mahmood N, Anjum FR, Amjad N, Shafique MS, and Ramzan A, 2022. Molecular detection of Salmonella gallinarum and its prevention using Autogenous Bacterin in white crystal neck commercial layer birds. J Microb Patho, 6(1):108-113. https://doi.org/10.37421/jmp.22.06.108

11. Asghar I, Rizvi F, Usmani MW, Shakir MZ, Mahmood N, Numan M, Ikram MS, and Waqar N, 2022. Immunomodulatory effect of turmeric (Curcuma longa) in Escherichia coli induced infected broiler chicks. J Microb Patho, 6(5):1-5. https://doi.org/10.37421/2684-4931.2022.6.129

Book Chapters

1. Usmani MW, Rizvi F, Shakir MZ, Mahmood N, Numan M, Abdullah RM, Tahir J, Shafiq MS, and Hameed HA, 2023. Factors influencing the emergence and re-emergence of zoonotic infectious diseases in livestock and human populations. In: Khan A, Abbas RZ, Hassan MF, Aguilar-Marcelino L, Saeed NM and Mohsin M (eds), Zoonosis, Unique Scientific Publishers, Faisalabad, Pakistan, 1:316-326. https://doi.org/10.47278/book.zoon/2023.023

2. Khan AMA, Arshad MA, Naeem RF, Shafiq MS, Irshad M, Shahid DA, Rizwan M, and MW Usmani, 2023. Role of essential oils and other alternatives to control ticks (Hyalomma species) the major cause of CCHF (a threat for humans and livestock). In: Complementary and Alternative Medicine: One Health Perspective (Sindhu ZuD, B Aslam, U Uslu & M Mohsin, eds): FahumSci, Lahore, Pakistan, 1:38-46. https://doi.org/10.61748/CAM.2023/006

3. Khan A, Alvi MA, Haq SU, Khan MF, Jan AW, Usmani MW, and Siddiqui SA, 2023.Zoonotic parasites and food safety: the case of Taenia solium. In: Khan A, Abbas RZ, Hassan MF, Aguilar-Marcelino L, Saeed NM and Mohsin M (eds), Zoonosis, Unique Scientific Publishers, Faisalabad, Pakistan, 2:275-288. https://doi.org/10.47278/book.zoon/2023.70

4. Imran M, Aslam M, Saleemi MK, Cao S, Ye J, Gul A-HST, Khatoon A, Usmani MW, Alvi MA, Nawaz S, Imran M, Naeem MA, and Jamil M, 2023. Zika Virus: A Pathological and Clinical Perspective. In: Aguilar-Marcelino L, Ababs RZ, Khan A, Younus M and Saeed NM (eds), One Health Triad, Unique Scientific Publishers, Faisalabad, 1: 121-127. https://doi.org/10.47278/book.oht/2023.19

5. Anjum FR, Anam S, Luqman M, Al-Surhanee AA, Shater AF, Usmani MW, Rahman SU, Sajid MS, Rizvi F, and Shakir MZ. Fungal Immunology: Mechanism of host innate immune recognition and evasion by pathogenic fungi. Fungal Reproduction and Growth. 2022. 1^st Edition. IntechOpen Publisher, London, UK. https://doi.org/10.5772/intechopen.101415

Gene Sequence/NCBI

1. Usmani MW, Rizvi F, Khatoon A, and Mahmood MS. Buffalopox virus isolate PAK-UAF (1 – 10) C18L-like gene, partial sequence (sanger dideoxy sequencing). OQ926872 – OQ926881 (339bp, DNA linear).

2. Mahmood N, Rizvi F, Saleemi MK, Shakir MZ, and Usmani MW. Salmonella enterica subsp. enterica serovar Pullorum isolate NM1 glucose-1-phosphate adenylyl transferase (glgC) pseudogene, partial sequence. MW550074 (249bp, DNA linear).

3. Shakir MZ, Rizvi F, Arshad MI, Mahmood N, and Usmani MW. Salmonella enterica subsp. enterica serovar Gallinarum isolate MZS1 glucose-1-phosphate adenylyl transferase (glgC) pseudogene, partial sequence. MW550075 (249bp, DNA linear).